X-ray free-electron laser studies reveal correlated motion during isopenicillin N synthase catalysis

Author:

Rabe Patrick1ORCID,Kamps Jos J. A. G.123ORCID,Sutherlin Kyle D.4ORCID,Linyard James D. S.1,Aller Pierre23,Pham Cindy C.4,Makita Hiroki4,Clifton Ian1ORCID,McDonough Michael A.1,Leissing Thomas M.1,Shutin Denis1,Lang Pauline A.1ORCID,Butryn Agata23,Brem Jürgen1ORCID,Gul Sheraz4ORCID,Fuller Franklin D.5,Kim In-Sik4,Cheah Mun Hon6ORCID,Fransson Thomas7ORCID,Bhowmick Asmit4,Young Iris D.48ORCID,O’Riordan Lee4ORCID,Brewster Aaron S.4ORCID,Pettinati Ilaria1ORCID,Doyle Margaret4,Joti Yasumasa910,Owada Shigeki910ORCID,Tono Kensuke910ORCID,Batyuk Alexander5ORCID,Hunter Mark S.5,Alonso-Mori Roberto5ORCID,Bergmann Uwe1112ORCID,Owen Robin L.2ORCID,Sauter Nicholas K.4ORCID,Claridge Timothy D. W.1ORCID,Robinson Carol V.1ORCID,Yachandra Vittal K.4ORCID,Yano Junko4ORCID,Kern Jan F.4ORCID,Orville Allen M.23ORCID,Schofield Christopher J.1ORCID

Affiliation:

1. Chemistry Research Laboratory, Department of Chemistry and the Ineos Oxford Institute for Antimicrobial Research, University of Oxford, 12 Mansfield Road, Oxford OX1 3TA, UK.

2. Diamond Light Source, Diamond House, Harwell Science and Innovation Campus, Didcot OX11 0DE, UK.

3. Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot, Oxfordshire OX11 0FA, UK.

4. Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA 94720, USA.

5. Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.

6. Department of Chemistry - Ångström, Molecular Biomimetics, Uppsala University, SE 751 20 Uppsala, Sweden.

7. Interdisciplinary Center for Scientific Computing, University of Heidelberg, 69120 Heidelberg, Germany.

8. Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, 600 16th Street, San Francisco, CA 94158, USA.

9. Japan Synchrotron Radiation Research Institute, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5198, Japan.

10. RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148, Japan.

11. Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.

12. Department of Physics, University of Wisconsin–Madison, 1150 University Avenue, Madison, WI 53706, USA.

Abstract

Complementary XFEL methods reveal O 2 reaction intermediates and global protein dynamics during Isopenicillin N synthase catalysis.

Funder

National Institutes of Health

U.S. Department of Energy

Wellcome

SLAC National Accelerator Laboratory

Engineering and Physical Sciences Research Council

Medical Research Foundation

Alzheimer Nadační Fond

Cancer Research UK

Wellcome Trust Centre for Mitochondrial Research

Biotechnology and Biological Sciences Research Council

Deutsche Akademie der Naturforscher Leopoldina - Nationale Akademie der Wissenschaften

Sub ngstrom Compact Light 827 Source SACLA Japan The Rayonix

Ineos Oxford Institute

Royal Society Wolfson Fellowship

EPSRC Synthesis for Biology and Medicine CDT

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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