A simple, time- and cost-effective, high-throughput depletion strategy for deep plasma proteomics-Reference-Cited by-同舟云学术

A simple, time- and cost-effective, high-throughput depletion strategy for deep plasma proteomics

Published:2023-03-31 Issue:13 Volume:9 Page:
ISSN:2375-2548
Container-title:Science Advances
language:en
Short-container-title:Sci. Adv.

Author:

Viode Arthur¹²^ORCID,van Zalm Patrick¹²³^ORCID,Smolen Kinga K.²⁴^ORCID,Fatou Benoit¹²^ORCID,Stevenson David¹,Jha Meenakshi¹^ORCID,Levy Ofer²⁴⁵^ORCID,Steen Judith³⁶^ORCID,Steen Hanno¹²⁴^ORCID,

Affiliation:

1. Department of Pathology, Boston Children’s Hospital, Boston, MA, USA.

2. Harvard Medical School, Boston, MA, USA.

3. Department of Neuropsychology and Psychopharmacology, EURON, Faculty of Psychology and Neuroscience, Maastricht University, Maastricht, Netherlands.

4. Precision Vaccines Program, Division of Infectious Diseases, Boston Children’s Hospital, Boston, MA, USA.

5. Broad Institute of MIT and Harvard, Cambridge, MA, USA.

6. F. M. Kirby Neurobiology Center, Boston Children's Hospital, Boston, MA, USA.

Abstract

We introduce a cost-effective, robust high-throughput–compatible plasma depletion method enabling in-depth profiling of plasma that detects >1300 proteins per run with a throughput of 60 samples per day. The method has been fully validated by processing >3000 samples with no apparent batch effect at a cost for the depletion step of ~$2.5 per sample.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Link

https://www.science.org/doi/pdf/10.1126/sciadv.adf9717

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