Directed evolution of an α1,3-fucosyltransferase using a single-cell ultrahigh-throughput screening method-Reference-Cited by-同舟云学术

Directed evolution of an α1,3-fucosyltransferase using a single-cell ultrahigh-throughput screening method

Published:2019-10-04 Issue:10 Volume:5 Page:
ISSN:2375-2548
Container-title:Science Advances
language:en
Short-container-title:Sci. Adv.

Author:

Tan Yumeng¹^ORCID,Zhang Yong¹^ORCID,Han Yunbin¹²,Liu Hao¹,Chen Haifeng¹^ORCID,Ma Fuqiang¹³^ORCID,Withers Stephen G.⁴,Feng Yan¹^ORCID,Yang Guangyu¹^ORCID

Affiliation:

1. State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.

2. State Key Laboratory Cultivation Base for TCM Quality and Efficacy, College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China.

3. CAS Key Laboratory of Biomedical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, China.

4. Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, British Columbia V6T 1Z1, Canada.

Abstract

We report an efficient tool for engineering enzymes to economically produce fucosylated drugs and prebiotic supplements.

Funder

National Natural Science Foundation of China

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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