Ultrarapid cryo-arrest of living cells on a microscope enables multiscale imaging of out-of-equilibrium molecular patterns

Author:

Huebinger Jan1ORCID,Grecco Hernan2ORCID,Masip Martín E.1ORCID,Christmann Jens1ORCID,Fuhr Günter R.3,Bastiaens Philippe I. H.14ORCID

Affiliation:

1. Department of Systemic Cell Biology, Max Planck Institute of Molecular Physiology, Otto-Hahn-Str.11, 44227 Dortmund, Germany.

2. Department of Physics, FCEN, University of Buenos Aires and IFIBA, CONICET, Buenos Aires, Argentina.

3. Fraunhofer Institute for Biomedical Engineering, Joseph-von-Fraunhofer-Weg 1, 66280 Sulzbach, Germany.

4. Faculty of Chemistry and Chemical Biology, TU Dortmund University, Otto-Hahn-Str. 6, 44227 Dortmund, Germany.

Abstract

Fluorescence imaging under cryo-arrest allows resolution of dynamic signaling system features that are otherwise obscured.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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