Fast widefield imaging of neuronal structure and function with optical sectioning in vivo

Author:

Li Ziwei12ORCID,Zhang Qinrong1ORCID,Chou Shih-Wei3,Newman Zachary3ORCID,Turcotte Raphaël1ORCID,Natan Ryan1ORCID,Dai Qionghai2,Isacoff Ehud Y.345,Ji Na1346ORCID

Affiliation:

1. Department of Physics, University of California, Berkeley, Berkeley, CA 94720, USA.

2. Department of Automation, Tsinghua University, Beijing 100084, China.

3. Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.

4. Helen Wills Neuroscience Institute, University of California, Berkeley, Berkeley, CA 94720, USA.

5. Bioscience Division, Lawrence Berkeley National Laboratory, Berkeley, Berkeley, CA 94720, USA.

6. Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

Abstract

Adaptive optics and structured illumination enables fast and accurate imaging of neuronal structure and function in vivo.

Funder

National Institutes of Health

National Institute of Mental Health

Defense Advanced Research Projects Agency

China Scholarship Council

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference38 articles.

1. E. R. Kandel J. H. Schwartz T. M. Jessell S. A. Siegelbaum A. J. Hudspeth Principles of Neural Science Fifth Edition (McGraw-Hill Education 2012).

2. J. B. Pawley Handbook of Biological Confocal Microscopy (ed. 3 2008).

3. Optical sectioning microscopy

4. Deep tissue two-photon microscopy

5. Multiphoton Excitation Provides Optical Sections from Deeper within Scattering Specimens than Confocal Imaging

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