Coupling Cas9 to artificial inhibitory domains enhances CRISPR-Cas9 target specificity-Reference-Cited by-同舟云学术

Coupling Cas9 to artificial inhibitory domains enhances CRISPR-Cas9 target specificity

Published:2020-02-07 Issue:6 Volume:6 Page:
ISSN:2375-2548
Container-title:Science Advances
language:en
Short-container-title:Sci. Adv.

Author:

Aschenbrenner Sabine¹²³^ORCID,Kallenberger Stefan M.³⁴^ORCID,Hoffmann Mareike D.¹²,Huck Adrian¹^ORCID,Eils Roland³⁴^ORCID,Niopek Dominik¹⁴^ORCID

Affiliation:

1. Synthetic Biology Group, Institute for Pharmacy and Molecular Biotechnology (IPMB) and Center for Quantitative Analysis of Molecular and Cellular Biosystems (BioQuant), University of Heidelberg, Heidelberg 69120, Germany.

2. Division of Chromatin Networks, German Cancer Research Center (DKFZ), Heidelberg 69120, Germany.

3. Digital Health Center, Berlin Institute of Health (BIH) and Charité, Berlin 10178, Germany.

4. Health Data Science Unit, University Hospital Heidelberg, Heidelberg 69120, Germany.

Abstract

Fusing attenuated anti-CRISPR proteins to Cas9 improves specificity via kinetic insulation of ON- and OFF-target editing events.

Funder

Helmholtz Association

German research council

Federal Ministry of Education and Research

DKFZ, Heidelberg

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference41 articles.

1. A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity

2. RNA-Guided Human Genome Engineering via Cas9

3. Multiplex Genome Engineering Using CRISPR/Cas Systems

4. NmeCas9 is an intrinsically high-fidelity genome-editing platform

5. Genome-wide analysis reveals specificities of Cpf1 endonucleases in human cells

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