Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity-Reference-Cited by-全球学者库

Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity

Published:2017-08-04 Issue:8 Volume:3 Page:
ISSN:2375-2548
Container-title:Science Advances
language:en
Short-container-title:Sci. Adv.

Author:

Komor Alexis C.¹²³^ORCID,Zhao Kevin T.¹²³,Packer Michael S.¹²³^ORCID,Gaudelli Nicole M.¹²³^ORCID,Waterbury Amanda L.¹,Koblan Luke W.¹²³,Kim Y. Bill¹²³,Badran Ahmed H.¹²³,Liu David R.¹²³^ORCID

Affiliation:

1. Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.

2. Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02138, USA.

3. Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.

Abstract

Probing base editing outcomes leads to new C:G to T:A base editors with greater efficiency and product purity, and fewer indels.

Funder

Defense Sciences Office, DARPA

HHMI

NIBIB

NIGMS

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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5. Systematic quantification of HDR and NHEJ reveals effects of locus, nuclease, and cell type on genome-editing;Miyaoka Y.;Sci. Rep.,2016

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