Differentiation of PC12 Cells-Reference-Cited by-同舟云学术

Differentiation of PC12 Cells

Published:2006-09-05 Issue:351 Volume:2006 Page:
ISSN:1525-8882
Container-title:Science's STKE
language:en
Short-container-title:Sci. STKE

Author:

Adler Elizabeth M.¹,Gough Nancy R.²,Blundon Jay A.³

Affiliation:

1. Associate Editor of Science's STKE, American Association for the Advancement of Science, 1200 New York Avenue, N.W., Washington, DC 20005, USA.

2. Editor of Science's STKE, American Association for the Advancement of Science, 1200 New York Avenue, N.W., Washington, DC 20005, USA.

3. Department of Biology, Rhodes College, Memphis, TN 38112, USA.

Abstract

This 3-week-long series of collaborative laboratory exercises explores how to use a cultured cell system (PC12 cells) to study signaling pathways involved in cellular differentiation. The laboratory would be useful in a neurobiology or cell biology course for advanced undergraduate students. The background and details for performing the lab are provided along with suggestions for assessing student performance and understanding.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

General Medicine

Reference6 articles.

1. E. M. Adler, N. R. Gough, Assessing undergraduate laboratory performance. Sci. STKE 2006, tr8 (2006).

2. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor.

3. PC12 Pheochromocytoma Cultures in Neurobiological Research

4. T. M. Jessel S. Schacher "Control of cell identity " especially "The fate of neural crest cells is controlled by the local environment" in Principles of Neural Science E. R. Kandel J. H. Schwartz T. M. Jessel Eds. (Elsevier New York ed. 3. 1991) pp. 887–907.

5. The 38-amino acid form of pituitary adenylate cyclase-activating polypeptide stimulates dual signaling cascades in PC12 cells and promotes neurite outgrowth.

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