CRISPR-Cas9 delivery to hard-to-transfect cells via membrane deformation-Reference-Cited by-同舟云学术

CRISPR-Cas9 delivery to hard-to-transfect cells via membrane deformation

Published:2015-08-07 Issue:7 Volume:1 Page:
ISSN:2375-2548
Container-title:Science Advances
language:en
Short-container-title:Sci. Adv.

Author:

Han Xin¹²,Liu Zongbin¹²,Jo Myeong chan¹²,Zhang Kai¹²,Li Ying¹²^ORCID,Zeng Zihua³,Li Nan⁴,Zu Youli³,Qin Lidong¹²⁵

Affiliation:

1. Department of Nanomedicine, Houston Methodist Research Institute, Houston, TX 77030, USA.

2. Department of Cell and Developmental Biology, Weill Medical College of Cornell University, New York, NY 10065, USA.

3. Department of Pathology and Genomic Medicine and Cancer Pathology Laboratory, Houston Methodist Hospital and Houston Methodist Research Institute, Houston, TX 77030, USA.

4. Department of Experimental Radiation Oncology, The University of Texas M.D. Anderson Cancer Center, Houston, TX 77030, USA.

5. Department of Molecular and Cellular Oncology, The University of Texas M.D. Anderson Cancer Center, Houston, TX 77030, USA.

Abstract

Virus-free gene editing possible at chromosomes through stretching cells in a micropost array, even for hard-to-transfect cells.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference31 articles.

1. RNA-Guided Human Genome Engineering via Cas9

2. Multiplex Genome Engineering Using CRISPR/Cas Systems

3. Development and Applications of CRISPR-Cas9 for Genome Engineering

4. CRISPR-mediated direct mutation of cancer genes in the mouse liver

5. Genetic Screens in Human Cells Using the CRISPR-Cas9 System

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