Fast iodide-SAD phasing for high-throughput membrane protein structure determination

Author:

Melnikov Igor1,Polovinkin Vitaly234,Kovalev Kirill34ORCID,Gushchin Ivan34ORCID,Shevtsov Mikhail4,Shevchenko Vitaly345,Mishin Alexey4,Alekseev Alexey34,Rodriguez-Valera Francisco6ORCID,Borshchevskiy Valentin4,Cherezov Vadim47,Leonard Gordon A.1,Gordeliy Valentin234,Popov Alexander1

Affiliation:

1. European Synchrotron Radiation Facility, 38043 Grenoble, France.

2. Institut de Biologie Structurale Jean-Pierre Ebel, Université Grenoble Alpes–Commissariat à l'Energie Atomique et aux Energies Alternatives–CNRS, F-38000 Grenoble, France.

3. Institute of Complex Systems (ICS), ICS-6, Structural Biochemistry, Research Centre Jülich, 52425 Jülich, Germany.

4. Moscow Institute of Physics and Technology, 141700 Dolgoprudny, Moscow Region, Russia.

5. Institute of Crystallography, University of Aachen (RWTH), Aachen, Germany.

6. Department of Vegetal Production and Microbiology, Universidad Miguel Hernández de Elche, San Juan de Alicante, Valencia, Spain.

7. Departments of Chemistry, Biological Sciences, and Physics & Astronomy, Bridge Institute, University of Southern California, Los Angeles, CA 90089, USA.

Abstract

A potentially universal method for the de novo solution of the crystal structures of membrane proteins is described.

Funder

Russian Science Foundation

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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