Metagenomics to Paleogenomics: Large-Scale Sequencing of Mammoth DNA-Reference-Cited by-同舟云学术

Metagenomics to Paleogenomics: Large-Scale Sequencing of Mammoth DNA

Published:2006-01-20 Issue:5759 Volume:311 Page:392-394
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Poinar Hendrik N.¹²³⁴⁵,Schwarz Carsten¹²³⁴⁵,Qi Ji¹²³⁴⁵,Shapiro Beth¹²³⁴⁵,MacPhee Ross D. E.¹²³⁴⁵,Buigues Bernard¹²³⁴⁵,Tikhonov Alexei¹²³⁴⁵,Huson Daniel H.¹²³⁴⁵,Tomsho Lynn P.¹²³⁴⁵,Auch Alexander¹²³⁴⁵,Rampp Markus¹²³⁴⁵,Miller Webb¹²³⁴⁵,Schuster Stephan C.¹²³⁴⁵

Affiliation:

1. McMaster Ancient DNA Center, McMaster University, 1280 Main Street West, Hamilton ON, L8S 4L9 Canada.

2. Department of Anthropology, McMaster University, 1280 Main Street West, Hamilton ON, L8S 4L9 Canada.

3. Department of Pathology and Molecular Medicine, McMaster University, 1280 Main Street West, Hamilton ON, L8S 4L9 Canada.

4. Pennsylvania State University, Center for Comparative Genomics and Bioinformatics, 310 Wartik Building, University Park, PA 16802, USA.

5. Henry Wellcome Ancient Biomolecules Centre, Department of Zoology, Oxford University, South Parks Road, Oxford, OX1 3PS, UK.

Abstract

We sequenced 28 million base pairs of DNA in a metagenomics approach, using a woolly mammoth (Mammuthus primigenius) sample from Siberia. As a result of exceptional sample preservation and the use of a recently developed emulsion polymerase chain reaction and pyrosequencing technique, 13 million base pairs (45.4%) of the sequencing reads were identified as mammoth DNA. Sequence identity between our data and African elephant (Loxodonta africana) was 98.55%, consistent with a paleontologically based divergence date of 5 to 6 million years. The sample includes a surprisingly small diversity of environmental DNAs. The high percentage of endogenous DNA recoverable from this single mammoth would allow for completion of its genome, unleashing the field of paleogenomics.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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