Molecular Coproscopy: Dung and Diet of the Extinct Ground Sloth Nothrotheriops shastensis-Reference-Cited by-同舟云学术

Molecular Coproscopy: Dung and Diet of the Extinct Ground Sloth Nothrotheriops shastensis

Published:1998-07-17 Issue:5375 Volume:281 Page:402-406
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Poinar Hendrik N.¹,Hofreiter Michael¹,Spaulding W. Geoffrey¹,Martin Paul S.¹,Stankiewicz B. Artur¹,Bland Helen¹,Evershed Richard P.¹,Possnert Göran¹,Pääbo Svante¹

Affiliation:

1. H. N. Poinar, M. Hofreiter, S. Pääbo, Max-Planck-Institute for Evolutionary Anthropology and Zoological Institute, University of Munich, Luisenstrasse 14, D-80333 Munich, Germany. W. G. Spaulding, Dames & Moore, 7115 Amigo Street, Suite 110, Las Vegas, NV 89119, USA. P. S. Martin, Desert Laboratory, Department of Geosciences, University of Arizona, Tucson, AZ 85721, USA. B. A. Stankiewicz, H. Bland, R. P. Evershed, Biogeochemistry Research Center, Department of Geology, University of Bristol,...

Abstract

DNA from excrements can be amplified by means of the polymerase chain reaction. However, this has not been possible with ancient feces. Cross-links between reducing sugars and amino groups were shown to exist in a Pleistocene coprolite from Gypsum Cave, Nevada. A chemical agent, N -phenacylthiazolium bromide, that cleaves such cross-links made it possible to amplify DNA sequences. Analyses of these DNA sequences showed that the coprolite is derived from an extinct sloth, presumably the Shasta ground sloth Nothrotheriops shastensis . Plant DNA sequences from seven groups of plants were identified in the coprolite. The plant assemblage that formed part of the sloth's diet exists today at elevations about 800 meters higher than the cave.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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