Div-Seq: Single-nucleus RNA-Seq reveals dynamics of rare adult newborn neurons-Reference-Cited by-同舟云学术

Div-Seq: Single-nucleus RNA-Seq reveals dynamics of rare adult newborn neurons

Published:2016-08-26 Issue:6302 Volume:353 Page:925-928
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Habib Naomi¹²³,Li Yinqing¹²³⁴,Heidenreich Matthias¹²³,Swiech Lukasz¹²³,Avraham-Davidi Inbal¹,Trombetta John J.¹,Hession Cynthia¹,Zhang Feng¹²³⁵⁶,Regev Aviv¹⁷

Affiliation:

1. Broad Institute of MIT and Harvard, 415 Main Street, Cambridge, MA 02142, USA.

2. Stanley Center for Psychiatric Research, 75 Ames Street, Cambridge, MA 02142, USA.

3. McGovern Institute of Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

4. Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

5. Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

6. Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

7. Howard Hughes Medical Institute, Koch Institute of Integrative Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Abstract

Visualizing gene expression in nuclei Gene expression can vary greatly within a single cell. Using techniques that they developed for sequencing single nuclei and labeling proliferating cells in vivo, Habib et al. performed RNA sequencing of 1402 single nuclei from the adult mouse hippocampus. Combining this approach with a clustering algorithm for single-cell and -nucleus RNA sequencing data delineated specific cell types during cell differentiation and development. By providing polyadenylated RNA from nuclei alone, as opposed to cytoplasmic RNA, these methods open the application of single-cell transcriptomics to tissues in which individual cells are difficult to isolate. Science , this issue p. 925

Funder

Howard Hughes Medical Institute

Helen Hay Whitney Foundation

Human Frontier Science Program

Klarman Cell Observatory at the Broad Institute

National Institute of Mental Health

NIMH

NSF

New York Stem Cell

Simons

Paul G. Allen Family

Vallee Foundation