Comment on “Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics”-Reference-Cited by-同舟云学术

Comment on “Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics”

Published:2016-04-29 Issue:6285 Volume:352 Page:527-527
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Sahl Steffen J.¹,Balzarotti Francisco¹,Keller-Findeisen Jan¹,Leutenegger Marcel¹,Westphal Volker¹,Egner Alexander²,Lavoie-Cardinal Flavie³,Chmyrov Andriy⁴⁵,Grotjohann Tim¹,Jakobs Stefan¹⁶

Affiliation:

1. Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Göttingen, Germany.

2. Laser Laboratory Göttingen, Hans-Adolf-Krebs-Weg 1, 37077 Göttingen, Germany.

3. Institut Universitaire en Santé Mentale de Québec, Cellular and Molecular Neuroscience Research Axis, 2601 Chemin de la Canardière, Québec, G1J 2G3, Canada.

4. Helmholtz Zentrum München, Institute of Biological and Medical Imaging, Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany.

5. Technische Universität München, Chair for Biological Imaging, Ismaningerstrasse 22, 81675 München, Germany.

6. University of Göttingen Medical Faculty, Department of Neurology, Robert-Koch-Str. 40, 37075 Göttingen, Germany.

Abstract

Li et al . (Research Articles, 28 August 2015, aab3500) purport to present solutions to long-standing challenges in live-cell microscopy, reporting relatively fast acquisition times in conjunction with improved image resolution. We question the methods’ reliability to visualize specimen features at sub–100-nanometer scales, because the mandatory mathematical processing of the recorded data leads to artifacts that are either difficult or impossible to disentangle from real features. We are also concerned about the chosen approach of subjectively comparing images from different super-resolution methods, as opposed to using quantitative measures.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference17 articles.

1. Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics

2. Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy. SHORT COMMUNICATION

3. True optical resolution beyond the Rayleigh limit achieved by standing wave illumination

4. Saturated patterned excitation microscopy—a concept for optical resolution improvement

5. Imaging and writing at the nanoscale with focused visible light through saturable optical transitions

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