Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics-Reference-Cited by-同舟云学术

Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics

Published:2015-08-28 Issue:6251 Volume:349 Page:
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Li Dong¹,Shao Lin¹,Chen Bi-Chang¹,Zhang Xi²³,Zhang Mingshu²,Moses Brian⁴,Milkie Daniel E.⁴,Beach Jordan R.⁵,Hammer John A.⁵,Pasham Mithun⁶,Kirchhausen Tomas⁶,Baird Michelle A.⁵⁷,Davidson Michael W.⁷,Xu Pingyong²,Betzig Eric¹

Affiliation:

1. Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.

2. Key Laboratory of RNA Biology and Beijing Key Laboratory of Noncoding RNA, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.

3. College of Life Sciences, Central China Normal University, Wuhan 430079, Hubei, China.

4. Coleman Technologies, 5131 West Chester Pike, Newtown Square, PA 19073, USA.

5. Cell Biology and Physiology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.

6. Department of Cell Biology and Pediatrics, Harvard Medical School and Program in Cellular and Molecular Medicine, Boston Children’s Hospital, Boston, MA 02115, USA.

7. National High Magnetic Field Laboratory and Department of Biological Science, Florida State University, Tallahassee, FL 32310, USA.

Abstract

Adding to the super-resolution arsenal Structured illumination microscopy (SIM) uses light intensities that are orders of magnitude lower than other super-resolution methods. SIM is also far faster over cellular-sized fields of view. Li et al. used two approaches to improve the resolution of SIM to allow live cell imaging of dynamic cellular processes, including endocytosis and cytoskeleton remodeling. The contrast in performance between SIM and other techniques is due to a few key differences. Defining the practical resolution at the limited signal-to-noise ratios necessary for live cell imaging will require better imaging metrics. Science , this issue 10.1126/science.aab3500

Funder

NIH

Howard Hughes Medical Institute

National Natural Science Foundation of China

Beijing Natural Science Foundation, China

National Basic Research Program

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference67 articles.

1. A guide to super-resolution fluorescence microscopy