Assembly and Validation of the Genome of the Nonmodel Basal Angiosperm Amborella

Author:

Chamala Srikar1,Chanderbali Andre S.12,Der Joshua P.3,Lan Tianying4,Walts Brandon1,Albert Victor A.4,dePamphilis Claude W.3,Leebens-Mack Jim5,Rounsley Steve6,Schuster Stephan C.789,Wing Rod A.1011,Xiao Nianqing12,Moore Richard12,Soltis Pamela S.213,Soltis Douglas E.1213,Barbazuk W. Brad113

Affiliation:

1. Department of Biology, University of Florida, Gainesville, FL 32611, USA.

2. Florida Museum of Natural History, University of Florida, Gainesville, FL 32611, USA.

3. Department of Biology and Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA.

4. Department of Biological Sciences, University at Buffalo (State University of New York), Buffalo, NY 14260, USA.

5. Department of Plant Biology, University of Georgia, Athens, GA 30602, USA.

6. Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN 46268, USA.

7. Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA.

8. Center for Comparative Genomics and Bioinformatics, The Pennsylvania State University, University Park, PA 16802, USA.

9. Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, 637551 Singapore.

10. Arizona Genomics Institute, University of Arizona, Tucson, AZ 5721, USA.

11. School of Plant Sciences and BIO5 Institute for Collaborative Research, University of Arizona, Tucson, AZ 85721, USA.

12. OpGen, Inc., 708 Quince Orchard Road, Gaithersburg, MD 20878, USA.

13. Genetics Institute, University of Florida, Gainesville, FL 32610, USA.

Abstract

Shaping Plant Evolution Amborella trichopoda is understood to be the most basal extant flowering plant and its genome is anticipated to provide insights into the evolution of plant life on Earth (see the Perspective by Adams ). To validate and assemble the sequence, Chamala et al. (p. 1516 ) combined fluorescent in situ hybridization (FISH), genomic mapping, and next-generation sequencing. The Amborella Genome Project (p. 10.1126/science.1241089 ) was able to infer that a whole-genome duplication event preceded the evolution of this ancestral angiosperm, and Rice et al. (p. 1468 ) found that numerous genes in the mitochondrion were acquired by horizontal gene transfer from other plants, including almost four entire mitochondrial genomes from mosses and algae.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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