Production of α-1,3-Galactosyltransferase Knockout Pigs by Nuclear Transfer Cloning-Reference-Cited by-同舟云学术

Production of α-1,3-Galactosyltransferase Knockout Pigs by Nuclear Transfer Cloning

Published:2002-02-08 Issue:5557 Volume:295 Page:1089-1092
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Lai Liangxue¹,Kolber-Simonds Donna²,Park Kwang-Wook¹,Cheong Hee-Tae¹³,Greenstein Julia L.²,Im Gi-Sun¹⁴,Samuel Melissa¹,Bonk Aaron¹,Rieke August¹,Day Billy N.¹,Murphy Clifton N.¹,Carter David B.¹⁵,Hawley Robert J.²,Prather Randall S.¹

Affiliation:

1. Department of Animal Science,

2. Immerge BioTherapeutics Inc., Charlestown, MA 02129, USA.

3. Department of Veterinary Medicine, College of Animal Resource Science, Kangwon National University, Chunchon 200-701, Korea.

4. National Livestock Research Institute, Suwon 441-350, Korea.

5. Department of Veterinary Pathobiology, University of Missouri, Columbia, MO 65211, USA.

Abstract

The presence of galactose α-1,3-galactose residues on the surface of pig cells is a major obstacle to successful xenotransplantation. Here, we report the production of four live pigs in which one allele of the α-1,3-galactosyltransferase locus has been knocked out. These pigs were produced by nuclear transfer technology; clonal fetal fibroblast cell lines were used as nuclear donors for embryos reconstructed with enucleated pig oocytes.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference36 articles.

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2. DISCORDANT ORGAN XENOTRANSPLANTATION IN PRIMATES

3. Expression of the human α:1,2‐fucosyltransferase in transgenic pigs modifies the cell surface carbohydrate phenotype and confers resistance to human serum‐mediated cytolysis

4. Remodeling of the Major Pig Xenoantigen by N-Acetylglucosaminyltransferase III in Transgenic Pig

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