Requirement of Guanosine Triphosphate-Bound Ran for Signal-Mediated Nuclear Protein Export-Reference-Cited by-同舟云学术

Requirement of Guanosine Triphosphate-Bound Ran for Signal-Mediated Nuclear Protein Export

Published:1997-06-20 Issue:5320 Volume:276 Page:1842-1844
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Richards Stephanie A.¹²,Carey Kimberly L.¹²,Macara Ian G.¹²

Affiliation:

1. S. A. Richards and K. L. Carey, Department of Pathology, University of Vermont, Burlington, VT 05405, USA.

2. I. G. Macara, Center for Cell Signaling, Box 577, Health Sciences Center, University of Virginia, Charlottesville, VA 22908, USA.

Abstract

A leucine-rich nuclear export signal (NES) allows rapid export of proteins from cell nuclei. Microinjection studies revealed a role for the guanosine triphosphatase (GTPase) Ran in NES-mediated export. Nuclear injection of a Ran mutant (Thr 24 → Asn) blocked protein export but not import, whereas depletion of the Ran nucleotide exchange factor RCC1 blocked protein import but not export. However, injection of Ran GTPase-activating protein (RanGAP) into RCC1-depleted cell nuclei inhibited export. Coinjection with Ran mutants insensitive to RanGAP prevented this inhibition. Therefore, NES-mediated protein export appears to require a Ran-GTP complex but does not require Ran-dependent GTP hydrolysis.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference23 articles.

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3. Identification of a signal for rapid export of proteins from the nucleus

4. Fukuda M., Gotoh I., Gotoh Y., Nishida E., J. Biol. Chem. 271, 21124 (1996);

5. Murphy R., Wente S. R., Nature 383, 357 (1996);

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