Collection, Mapping, and Annotation of Over 28,000 cDNA Clones from
japonica
Rice
Author:
Kikuchi Shoshi, Satoh Kouji, Nagata Toshifumi, Kawagashira Nobuyuki, Doi Koji, Kishimoto Naoki, Yazaki Junshi, Ishikawa Masahiro, Yamada Hitomi, Ooka Hisako, Hotta Isamu, Kojima Keiichi, Namiki Takahiro, Ohneda Eisuke, Yahagi Wataru, Suzuki Kohji, Li Chao Jie, Ohtsuki Kenji, Shishiki Toru, Otomo Yasuhiro, Murakami Kazuo, Iida Yoshiharu, Sugano Sumio, Fujimura Tatsuto, Suzuki Yutaka, Tsunoda Yuki, Kurosaki Takashi, Kodama Takeko, Masuda Hiromi, Kobayashi Michie, Xie Quihong, Lu Min, Narikawa Ryuya, Sugiyama Akio, Mizuno Kouichi, Yokomizo Satoko, Niikura Junko, Ikeda Rieko, Ishibiki Junya, Kawamata Midori, Yoshimura Akemi, Miura Junichirou, Kusumegi Takahiro, Oka Mitsuru, Ryu Risa, Ueda Mariko, Matsubara Kenichi, Kawai Jun1, Carninci Piero1, Adachi Jun1, Aizawa Katsunori1, Arakawa Takahiro1, Fukuda Shiro1, Hara Ayako1, Hashidume Wataru1, Hayatsu Norihito1, Imotani Koichi1, Ishii Yoshiki1, Itoh Masayoshi1, Kagawa Ikuko1, Kondo Shinji1, Konno Hedeaki1, Miyazaki Ai1, Osato Naoki1, Ota Yoshimi1, Saito Rintaro1, Sasaki Daisuke1, Sato Kenjiro1, Shibata Kazuhiro1, Shinagawa Akira1, Shiraki Toshiyuki1, Yoshino Masayasu1, Hayashizaki Yoshihide1
Affiliation:
1. Department of Molecular Biology, National Institute of Agrobiological Sciences, 2-1-2 Kannon-dai, Tsukuba, Ibaraki 305-8602, Japan. Hitachi Software Engineering Co., Ltd., 6-81, Onoe-cho, Naka-ku, Yokohama 231-0015, Japan. Laboratory of Genome Sequencing & Analysis Group, Foundation of Advancement of International Science (FAIS), 586-9, Akatsuka-Ushigahuchi, Tsukuba, Ibaraki 305-0062, Japan. Department of Virology, Institute of Medical Science, The University of Tokyo, 4-6-1, Shiroganedai,...
Abstract
We collected and completely sequenced 28,469 full-length complementary DNA clones from
Oryza sativa
L. ssp.
japonica
cv. Nipponbare. Through homology searches of publicly available sequence data, we assigned tentative protein functions to 21,596 clones (75.86%). Mapping of the cDNA clones to genomic DNA revealed that there are 19,000 to 20,500 transcription units in the rice genome. Protein informatics analysis against the InterPro database revealed the existence of proteins presented in rice but not in
Arabidopsis
. Sixty-four percent of our cDNAs are homologous to
Arabidopsis
proteins.
Publisher
American Association for the Advancement of Science (AAAS)
Subject
Multidisciplinary
Cited by
782 articles.
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