Electrode-Reducing Microorganisms That Harvest Energy from Marine Sediments-Reference-Cited by-同舟云学术

Electrode-Reducing Microorganisms That Harvest Energy from Marine Sediments

Published:2002-01-18 Issue:5554 Volume:295 Page:483-485
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Bond Daniel R.¹,Holmes Dawn E.¹,Tender Leonard M.²,Lovley Derek R.¹

Affiliation:

1. Department of Microbiology, University of Massachusetts, Amherst, MA 01003, USA.

2. Center for Bio/molecular Science and Engineering, Code 6900, Naval Research Laboratory, Washington, DC 20375, USA.

Abstract

Energy in the form of electricity can be harvested from marine sediments by placing a graphite electrode (the anode) in the anoxic zone and connecting it to a graphite cathode in the overlying aerobic water. We report a specific enrichment of microorganisms of the family Geobacteraceae on energy-harvesting anodes, and we show that these microorganisms can conserve energy to support their growth by oxidizing organic compounds with an electrode serving as the sole electron acceptor. This finding not only provides a method for extracting energy from organic matter, but also suggests a strategy for promoting the bioremediation of organic contaminants in subsurface environments.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference26 articles.

1. Harvesting Energy from the Marine Sediment−Water Interface

2. Electrodes used in sediment incubations were solid unpolished graphite with a total surface area of 62.8 cm 2 . Connections were made with threaded marine-grade water-tight connectors filled with silver epoxy and sealed with marine epoxy. Sediments and seawater were from Boston Harbor MA.

3. Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses.

4. Design and Evaluation of Useful Bacterium-Specific PCR Primers That Amplify Genes Coding for Bacterial 16S rRNA

5. Electrodes were rinsed under a stream of sterile marine buffer to remove all visible sediment and debris then scraped with sterile razor blades to remove adherent bacteria. DNA was extracted from this graphite slurry by a standard lysozyme-SDS–hexadecyltrimethyl ammonium bromide (CTAB) phenol-chloroform method and further cleaned using a Wizard DNA purification kit (Promega). DNA was independently extracted from electrodes used in four different incubations.

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