Role of Importin-β in Coupling Ran to Downstream Targets in Microtubule Assembly

Abstract

The guanosine triphosphatase Ran stimulates assembly of microtubule asters and spindles in mitotic Xenopus egg extracts. A carboxyl-terminal region of the nuclear-mitotic apparatus protein (NuMA), a nuclear protein required for organizing mitotic spindle poles, mimics Ran's ability to induce asters. This NuMA fragment also specifically interacted with the nuclear transport factor, importin-β. We show that importin-β is an inhibitor of microtubule aster assembly in Xenopus egg extracts and that Ran regulates the interaction between importin-β and NuMA. Importin-β therefore links NuMA to regulation by Ran. This suggests that similar mechanisms regulate nuclear import during interphase and spindle assembly during mitosis.