Affiliation:
1. Carnegie Institution of Washington, Department of Embryology, Baltimore, MD 21210, USA.
Abstract
Ran, a small guanosine triphosphatase, is suggested to have additional functions beyond its well-characterized role in nuclear trafficking. Guanosine triphosphate–bound Ran, but not guanosine diphosphate–bound Ran, stimulated polymerization of astral microtubules from centrosomes assembled on
Xenopus
sperm. Moreover, a Ran allele with a mutation in the effector domain (RanL43E) induced the formation of microtubule asters and spindle assembly, in the absence of sperm nuclei, in a γTuRC (γ-tubulin ring complex)– and XMAP215 (
Xenopus
microtubule associated protein)–dependent manner. Therefore, Ran could be a key signaling molecule regulating microtubule polymerization during mitosis.
Publisher
American Association for the Advancement of Science (AAAS)
Reference29 articles.
1. Verde F., Dogterom M., Stelzer E., Karsenti E., Leibler S., J. Cell Biol. 118, 1097 (1992).
2. Verde F., Labbe J., Doree M., Karsenti E., Nature 343, 233 (1990).
3. Zhang D., Nicklas R., J. Cell Biol. 131, 1125 (1995).
4. Dogterom M., Felix M., Guet C., Leibler S., ibid. 133, 125 (1996).
5. E. Karsenti J. Newport R. Hubble M. W. Kirschner ibid. 98 1730 (1984).
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