Multiplex recording of cellular events over time on CRISPR biological tape-Reference-Cited by-同舟云学术

Multiplex recording of cellular events over time on CRISPR biological tape

Published:2017-12-15 Issue:6369 Volume:358 Page:1457-1461
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Sheth Ravi U.¹²^ORCID,Yim Sung Sun¹^ORCID,Wu Felix L.¹²^ORCID,Wang Harris H.¹³^ORCID

Affiliation:

1. Department of Systems Biology, Columbia University, New York, NY, USA.

2. Integrated Program in Cellular, Molecular, and Biomedical Studies, Columbia University, New York, NY, USA.

3. Department of Pathology and Cell Biology, Columbia University, New York, NY, USA.

Abstract

A CRISPR device to record time The CRISPR adaptation system has been used to record the sequence and ordering of exogenous oligonucleotides that are electroporated into cell populations. Sheth et al. engineered a system bypassing the use of exogenous DNA to directly record temporal signals. An input biological signal is transformed into the ratio of the frequency of incorporating trigger DNA to that of incorporating reference DNA into the genomes of a bacterial population. A multiplexing strategy enables simultaneous recording of three environmental signals with high temporal resolution. Science , this issue p. 1457

Funder

National Science Foundation

National Institutes of Health

Office of Naval Research

Alfred P. Sloan Foundation

Hertz Foundation

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference44 articles.

1. Synthetic Gene Networks That Count