Spatially resolved single-cell translatomics at molecular resolution-Reference-Cited by-同舟云学术

Spatially resolved single-cell translatomics at molecular resolution

Published:2023-06-30 Issue:6652 Volume:380 Page:
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Zeng Hu¹²^ORCID,Huang Jiahao¹²^ORCID,Ren Jingyi¹²^ORCID,Wang Connie Kangni²^ORCID,Tang Zefang¹²^ORCID,Zhou Haowen²^ORCID,Zhou Yiming¹²³^ORCID,Shi Hailing¹²^ORCID,Aditham Abhishek²⁴^ORCID,Sui Xin¹²^ORCID,Chen Hongyu¹²^ORCID,Lo Jennifer A.²^ORCID,Wang Xiao¹²^ORCID

Affiliation:

1. Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

2. Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.

3. Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.

4. Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Abstract

The precise control of messenger RNA (mRNA) translation is a crucial step in posttranscriptional gene regulation of cellular physiology. However, it remains a challenge to systematically study mRNA translation at the transcriptomic scale with spatial and single-cell resolution. Here, we report the development of ribosome-bound mRNA mapping (RIBOmap), a highly multiplexed three-dimensional in situ profiling method to detect cellular translatome. RIBOmap profiling of 981 genes in HeLa cells revealed cell cycle–dependent translational control and colocalized translation of functional gene modules. We mapped 5413 genes in mouse brain tissues, yielding spatially resolved single-cell translatomic profiles for 119,173 cells and revealing cell type–specific and brain region–specific translational regulation, including translation remodeling during oligodendrocyte maturation. Our method detected widespread patterns of localized translation in neuronal and glial cells in intact brain tissue networks.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Link

https://www.science.org/doi/pdf/10.1126/science.add3067

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