Pig Cloning by Microinjection of Fetal Fibroblast Nuclei-Reference-Cited by-同舟云学术

Pig Cloning by Microinjection of Fetal Fibroblast Nuclei

Published:2000-08-18 Issue:5482 Volume:289 Page:1188-1190
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Onishi Akira¹,Iwamoto Masaki²,Akita Tomiji¹,Mikawa Satoshi¹,Takeda Kumiko¹,Awata Takashi¹,Hanada Hirohumi¹,Perry Anthony C. F.³

Affiliation:

1. Department of Animal Breeding and Genetics, National Institute of Animal Industry, Tsukuba Norin Danchi, Ibaraki-ken 305–0901, Japan.

2. Basic Research Division, Prima Meatpackers, Tsuchiura-shi, Ibaraki-ken 300–0841, Japan.

3. Laboratory of Developmental Biology, The Rockefeller University, New York, NY 10021, USA.

Abstract

Pig cloning will have a marked impact on the optimization of meat production and xenotransplantation. To clone pigs from differentiated cells, we microinjected the nuclei of porcine ( Sus scrofa ) fetal fibroblasts into enucleated oocytes, and development was induced by electroactivation. The transfer of 110 cloned embryos to four surrogate mothers produced an apparently normal female piglet. The clonal provenance of the piglet was indicated by her coat color and confirmed by DNA microsatellite analysis.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference36 articles.

1. Sheep cloned by nuclear transfer from a cultured cell line

2. Eight Calves Cloned from Somatic Cells of a Single Adult

3. Production of goats by somatic cell nuclear transfer

4. Nuclear transplantation in sheep embryos

5. Prather R. S., et al., Biol. Reprod. 41, 123 (1989).

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