Opening and Closing of the Bacterial RNA Polymerase Clamp-Reference-Cited by-同舟云学术

Opening and Closing of the Bacterial RNA Polymerase Clamp

Published:2012-08-03 Issue:6094 Volume:337 Page:591-595
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Chakraborty Anirban¹,Wang Dongye¹,Ebright Yon W.¹,Korlann You²,Kortkhonjia Ekaterine¹²,Kim Taiho³,Chowdhury Saikat⁴,Wigneshweraraj Sivaramesh⁵,Irschik Herbert⁶,Jansen Rolf⁶,Nixon B. Tracy⁴,Knight Jennifer⁷,Weiss Shimon²,Ebright Richard H.¹

Affiliation:

1. Howard Hughes Medical Institute, Waksman Institute, and Department of Chemistry and Chemical Biology, Rutgers University, Piscataway, NJ 08854, USA.

2. Department of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, CA 90095, USA.

3. Nesher Technologies, Inc., 2100 West Third Street, Los Angeles, CA 90057, USA.

4. Department of Biochemistry, Pennsylvania State University, State College, PA 16802, USA.

5. Centre for Molecular Microbiology and Infection, Imperial College London, London SW7 2AZ, UK.

6. Helmholtz Centre for Infection Research, 38124 Braunschweig, Germany.

7. Department of Chemistry, University of Michigan, Ann Arbor, MI 48109, USA.

Abstract

Clamping Down Crystal structures of RNA polymerase show that a “clamp” region which surrounds the DNA binding site can adopt conformations ranging from a closed to an open state. Chakraborty et al. (p. 591 ) used single-molecule fluorescence energy transfer experiments to detect the clamp's conformational changes in solution during the transcription cycle. The results support a model in which a clamp opening allows DNA to be loaded into the active-center cleft and unwound. Direct interactions with DNA likely trigger clamp closure upon formation of a catalytically competent transcription initiation complex.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference56 articles.

1. Crystal Structure of Thermus aquaticus Core RNA Polymerase at 3.3 Å Resolution

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3. Structural Basis of Transcription: An RNA Polymerase II Elongation Complex at 3.3 Å Resolution

4. Structural Basis of Transcription Initiation: RNA Polymerase Holoenzyme at 4 Å Resolution

5. Crystal structure of a bacterial RNA polymerase holoenzyme at 2.6 Å resolution

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