Budding Yeast Cdc20: A Target of the Spindle Checkpoint-Reference-Cited by-同舟云学术

Budding Yeast Cdc20: A Target of the Spindle Checkpoint

Published:1998-02-13 Issue:5353 Volume:279 Page:1041-1044
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Hwang Lena H.¹²,Lau Lucius F.¹²,Smith Dana L.¹²,Mistrot Cathy A.¹²,Hardwick Kevin G.¹²,Hwang Ellen S.¹²,Amon Angelika¹²,Murray Andrew W.¹²

Affiliation:

1. L. H. Hwang, D. L. Smith, C. A. Mistrot, K. G. Hardwick, A. W. Murray, Departments of Physiology and Biochemistry, University of California at San Francisco, San Francisco, CA 94143–0444, USA.

2. L. F. Lau, E. S. Hwang, A. Amon, Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA.

Abstract

The spindle checkpoint regulates the cell division cycle by keeping cells with defective spindles from leaving mitosis. In the two-hybrid system, three proteins that are components of the checkpoint, Mad1, Mad2, and Mad3, were shown to interact with Cdc20, a protein required for exit from mitosis. Mad2 and Mad3 coprecipitated with Cdc20 at all stages of the cell cycle. The binding of Mad2 depended on Mad1 and that of Mad3 on Mad1 and Mad2. Overexpression of Cdc20 allowed cells with a depolymerized spindle or damaged DNA to leave mitosis but did not overcome the arrest caused by unreplicated DNA. Mutants in Cdc20 that were resistant to the spindle checkpoint no longer bound Mad proteins, suggesting that Cdc20 is the target of the spindle checkpoint.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference36 articles.

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5. Activation of the Budding Yeast Spindle Assembly Checkpoint Without Mitotic Spindle Disruption

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