Polymorphic Secreted Kinases Are Key Virulence Factors in Toxoplasmosis-Reference-Cited by-同舟云学术

Polymorphic Secreted Kinases Are Key Virulence Factors in Toxoplasmosis

Published:2006-12-15 Issue:5806 Volume:314 Page:1780-1783
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Saeij J. P. J.¹²³,Boyle J. P.¹²³,Coller S.¹²³,Taylor S.¹²³,Sibley L. D.¹²³,Brooke-Powell E. T.¹²³,Ajioka J. W.¹²³,Boothroyd J. C.¹²³

Affiliation:

1. Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA.

2. Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA.

3. Department of Pathology, Cambridge University, Cambridge, CB2 1QP, UK.

Abstract

The majority of known Toxoplasma gondii isolates from Europe and North America belong to three clonal lines that differ dramatically in their virulence, depending on the host. To identify the responsible genes, we mapped virulence in F 1 progeny derived from crosses between type II and type III strains, which we introduced into mice. Five virulence ( VIR ) loci were thus identified, and for two of these, genetic complementation showed that a predicted protein kinase (ROP18 and ROP16, respectively) is the key molecule. Both are hypervariable rhoptry proteins that are secreted into the host cell upon invasion. These results suggest that secreted kinases unique to the Apicomplexa are crucial in the host-pathogen interaction.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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