A lineage-resolved molecular atlas of C. elegans embryogenesis at single-cell resolution-Reference-Cited by-同舟云学术

A lineage-resolved molecular atlas of C. elegans embryogenesis at single-cell resolution

Published:2019-09-20 Issue:6459 Volume:365 Page:
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Packer Jonathan S.¹^ORCID,Zhu Qin²^ORCID,Huynh Chau¹,Sivaramakrishnan Priya³^ORCID,Preston Elicia³^ORCID,Dueck Hannah³^ORCID,Stefanik Derek⁴^ORCID,Tan Kai³⁵⁶⁷^ORCID,Trapnell Cole¹^ORCID,Kim Junhyong⁴^ORCID,Waterston Robert H.¹^ORCID,Murray John I.³^ORCID

Affiliation:

1. Department of Genome Sciences, University of Washington, Seattle, WA, USA.

2. Genomics and Computational Biology Graduate Group, University of Pennsylvania, Philadelphia, PA, USA.

3. Department of Genetics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.

4. Department of Biology, University of Pennsylvania, Philadelphia, PA, USA.

5. Division of Oncology and Center for Childhood Cancer Research, Children’s Hospital of Philadelphia, Philadelphia, PA, USA.

6. Department of Pediatrics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.

7. Department of Cell and Developmental Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.

Abstract

Identifying terminal nematode cells Single-cell RNA sequencing provides the power to identify the developmental trajectory of an organism. However, identifying the temporal lineage of cell development can be difficult without large-scale analyses. Packer et al. sequenced more than 80,000 cells from embryos of the roundworm Caenorhabditis elegans to determine the expression of genes directing the development of terminal cell types. Because all somatic cells in a C. elegans individual have been mapped, the authors are able to connect gene expression with cell lineages over time during development, noting stark transitions in some cases. Science , this issue p. eaax1971

Funder

National Institutes of Health

National Cancer Institute

Commonwealth of Pennsylvania Health Research Formula Funds

William H. Gates Chair of Biomedical Sciences

Allen Discovery Center for Lineage Tracing

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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1. Single-cell mRNA quantification and differential analysis with Census