Kaposi's Sarcoma-Associated Herpesvirus Infection and Multiple Myeloma

Author:

Parravicini Carlo1,Lauri Eleonora1,Baldini Luca2,Neri Antonino2,Poli Francesca3,Sirchia Girolamo3,Moroni Mauro4,Galli Massimo4,Corbellino Mario4

Affiliation:

1. Servizio di Anatomia Patalogica, Luigi Sacco Hospital, 20157, Milano, Italy

2. Servizio di Ematologia, Centro Giovanni Marcora, Institute of Medical Sciences, University of Milan, Istituto di Ricerca e Cura a Carattere Scientifico (IRCCS) Ospedale Policlinico, Milano, Italy

3. North Italian Transplant, Centro Trasfusionale e di Immunologia dei Trapianti, IRCCS Ospedale Policlinico Milano, Italy

4. Institute of Infectious Diseases, University of Milan, Luigi Sacco Hospital, 20147, Milano, Italy E-mail:

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference65 articles.

1. Kaposi's Sarcoma-Associated Herpesvirus Infection of Bone Marrow Dendritic Cells from Multiple Myeloma Patients

2. Bone marrow mononuclear cells were obtained from bone marrow aspirates by Ficoll density centrifugation and their DNA extracted with the use of standard protocols [J. Sambrook et al. in Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory Press New York ed. 2 1989)]. Thirty-cycle PCR using human β-globin specific primers [

3. Bauer M., et al., J. Am. Med. Assoc. 256, 472 (1991);

4. ] and 1 μg of DNA as template was performed in order to rule out major Taq polymerase inhibitors and to verify the integrity of the extracted DNA. Nested PCR (35+35 amplification cycles) was performed as described in detail elsewhere [

5. Corbellino M., et al., AIDS Res. Hum. Retrovir. 12, 651 (1996);

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