An RNA Ligase Essential for RNA Editing and Survival of the Bloodstream Form of Trypanosoma brucei-Reference-Cited by-同舟云学术

An RNA Ligase Essential for RNA Editing and Survival of the Bloodstream Form of Trypanosoma brucei

Published:2001-03-16 Issue:5511 Volume:291 Page:2159-2162
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Schnaufer Achim¹²,Panigrahi Aswini K.¹²,Panicucci Brian¹,Igo Robert P.¹²,Salavati Reza¹²,Stuart Kenneth¹²

Affiliation:

1. Seattle Biomedical Research Institute, Seattle, WA 98109, USA.

2. Department of Pathobiology, University of Washington, Seattle, WA 98195, USA.

Abstract

RNA editing in trypanosomes occurs by a series of enzymatic steps that are catalyzed by a macromolecular complex. The TbMP52 protein is shown to be a component of this complex, to have RNA ligase activity, and to be one of two adenylatable proteins in the complex. Regulated repression of TbMP52 blocks editing, which shows that it is a functional component of the editing complex. This repression is lethal in bloodforms of the parasite, indicating that editing is essential in the mammalian stage of the life cycle. The editing complex, which is present in all kinetoplastid parasites, may thus be a chemotherapeutic target.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference26 articles.

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3. Uridine insertion/deletion RNA editing in trypanosome mitochondria — a review

4. Association of Two Novel Proteins, TbMP52 and TbMP48, with the Trypanosoma brucei RNA Editing Complex

5. Searches were done at www.ncbi.nlm.nih.gov/Structure/cdd/cdd.shtml.

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