Rationally engineered Cas9 nucleases with improved specificity-Reference-Cited by-同舟云学术

Rationally engineered Cas9 nucleases with improved specificity

Published:2016-01 Issue:6268 Volume:351 Page:84-88
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Slaymaker Ian M.¹²³⁴,Gao Linyi¹⁴,Zetsche Bernd¹²³⁴,Scott David A.¹²³⁴,Yan Winston X.¹⁵⁶,Zhang Feng¹²³⁴

Affiliation:

1. Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.

2. McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

3. Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

4. Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

5. Graduate Program in Biophysics, Harvard Medical School, Boston, MA 02115, USA.

6. Harvard-MIT Division of Health Sciences and Technology, Harvard Medical School, Boston, MA 02115, USA.

Abstract

Making the correct cut The CRISPR/Cas system is a prokaryotic immune system that targets and cuts out foreign DNA in bacteria. It has been adopted for gene editing because it can be designed to recognize and cut specific locations in the genome. A challenge in developing clinical applications is the potential for off-target effects that could result in DNA cleavage at the wrong locations. Slaymaker et al. used structure-guided engineering to improve the specificity of Streptococcus pyogenes Cas9 (SpCas9). They identified enhanced-specificity variants (eSpCas9) that display reduced off-target cleavage while maintaining robust on-target activity Science , this issue p. 84

Funder

National Institute of General Medical Sciences

Daisy Soros Fellowship

National Institutes of Health

NIMH

NIDDK

National Science Foundation

Keck

New York Stem Cell

Damon Runyon

Searle Scholars

Merkin