The widespread IS200/IS605 transposon family encodes diverse programmable RNA-guided endonucleases-Reference-Cited by-同舟云学术

The widespread IS200/IS605 transposon family encodes diverse programmable RNA-guided endonucleases

Published:2021-10 Issue:6563 Volume:374 Page:57-65
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Altae-Tran Han¹²³⁴⁵^ORCID,Kannan Soumya¹²³⁴⁵^ORCID,Demircioglu F. Esra¹²³⁴⁵^ORCID,Oshiro Rachel¹²³⁴⁵^ORCID,Nety Suchita P.¹²³⁴⁵^ORCID,McKay Luke J.⁶⁷⁸^ORCID,Dlakić Mensur⁹^ORCID,Inskeep William P.⁶⁷^ORCID,Makarova Kira S.¹⁰^ORCID,Macrae Rhiannon K.¹²³⁴⁵^ORCID,Koonin Eugene V.¹⁰^ORCID,Zhang Feng¹²³⁴⁵^ORCID

Affiliation:

1. Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

2. Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.

3. McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

4. Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

5. Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

6. Department of Land Resources and Environmental Sciences, Montana State University, Bozeman, MT 59717, USA.

7. Thermal Biology Institute, Montana State University, Bozeman, MT 59717, USA.

8. Center for Biofilm Engineering, Montana State University, Bozeman, MT 59717, USA.

9. Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT 59717, USA.

10. National Center for Biotechnology Information, National Library of Medicine, Bethesda, MD 20894, USA.

Abstract

Tracing the origin of CRISPR-Cas CRISPR-Cas systems have transformed genome editing and other biotechnologies; however, the broader origins and diversity of RNA-guided nucleases have largely remained unexplored. Altae-Tran et al . show that three distinct transposon-encoded proteins, IscB, IsrB, and TnpB, are naturally occurring, reprogrammable RNA-guided DNA nucleases (see the Perspective by Rousset and Sorek). In addition to identifying diverse guide-encoding mechanisms, the authors elucidate the evolutionary relationship between IsrB, IscB, and CRISPR-Cas9. Overall, these newly characterized systems, called OMEGA (for obligate mobile element–guided activity) systems, are found in all domains of life and may be harnessed for biotechnology development. —DJ

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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