Affiliation:
1. Cancer Research Campaign Cell Cycle Genetics Laboratories, Medical Sciences Institute, University of Dundee, Dundee DD1 4HN, UK.
Abstract
The product of the
abnormal spindle
(
asp
) gene was found to be an asymmetrically localized component of the centrosome during mitosis, required to focus the poles of the mitotic spindle in vivo. Removing Asp protein function from
Drosophila melanogaster
embryo extracts, either by mutation or immunodepletion, resulted in loss of their ability to restore microtubule-organizing center activity to salt-stripped centrosome preparations. This was corrected by addition of purified Asp protein. Thus, Asp appears to hold together the microtubule-nucleating γ-tubulin ring complexes that organize the mitotic centrosome.
Publisher
American Association for the Advancement of Science (AAAS)
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