Bruchpilot Promotes Active Zone Assembly, Ca 2+ Channel Clustering, and Vesicle Release-Reference-Cited by-同舟云学术

Bruchpilot Promotes Active Zone Assembly, Ca 2+ Channel Clustering, and Vesicle Release

Published:2006-05-19 Issue:5776 Volume:312 Page:1051-1054
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Kittel Robert J.¹²³⁴⁵,Wichmann Carolin¹²³⁴⁵,Rasse Tobias M.¹²³⁴⁵,Fouquet Wernher¹²³⁴⁵,Schmidt Manuela¹²³⁴⁵,Schmid Andreas¹²³⁴⁵,Wagh Dhananjay A.¹²³⁴⁵,Pawlu Christian¹²³⁴⁵,Kellner Robert R.¹²³⁴⁵,Willig Katrin I.¹²³⁴⁵,Hell Stefan W.¹²³⁴⁵,Buchner Erich¹²³⁴⁵,Heckmann Manfred¹²³⁴⁵,Sigrist Stephan J.¹²³⁴⁵

Affiliation:

1. European Neuroscience Institute Göttingen, Grisebachstrasse 5, 37077 Göttingen, Germany.

2. Institut für Klinische Neurobiologie, Josef Schneider Strasse 11, 97080 Würzburg, Germany.

3. Lehrstuhl für Genetik und Neurobiologie, Am Hubland, 97074 Würzburg, Germany.

4. Department of Nano-Biophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany.

5. Institut für Klinische Neurobiologie, Rudolf Virchow Zentrum, 97080 Würzburg, Germany.

Abstract

The molecular organization of presynaptic active zones during calcium influx–triggered neurotransmitter release is the focus of intense investigation. The Drosophila coiled-coil domain protein Bruchpilot (BRP) was observed in donut-shaped structures centered at active zones of neuromuscular synapses by using subdiffraction resolution STED (stimulated emission depletion) fluorescence microscopy. At brp mutant active zones, electron-dense projections (T-bars) were entirely lost, Ca 2+ channels were reduced in density, evoked vesicle release was depressed, and short-term plasticity was altered. BRP-like proteins seem to establish proximity between Ca 2+ channels and vesicles to allow efficient transmitter release and patterned synaptic plasticity.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference38 articles.

1. R. G. Zhai, H. J. Bellen, Physiology (Bethesda)19, 262 (2004).

2. The effect of calcium on acetylcholine release from motor nerve terminals

3. Vesicle Pools and Ca2+ Microdomains: New Tools for Understanding Their Roles in Neurotransmitter Release

4. Diversification of synaptic strength: presynaptic elements

5. Drosophila larval neuromuscular junction: Molecular components and mechanisms underlying synaptic plasticity

Cited by 697 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Super-resolution microscopy to study membrane nanodomains and transport mechanisms in the plasma membrane;Frontiers in Molecular Biosciences;2024-09-03

2. Four SpsP neurons are an integrating sleep regulation hub inDrosophila;2024-09-03

3. Enhanced Performance of the Optimized Dye CF583R in Direct Stochastic Optical Reconstruction Microscopy of Active Zones in Drosophila Melanogaster;Cells;2024-08-28

4. Presynaptic neurons self-tune by inversely coupling neurotransmitter release with the abundance of CaV2 voltage-gated Ca ²⁺ channels;Proceedings of the National Academy of Sciences;2024-08-22

5. Distinct active zone protein machineries mediate Ca2+ channel clustering and vesicle priming at hippocampal synapses;Nature Neuroscience;2024-08-19