Affiliation:
1. Hubrecht Laboratory, Center for Biomedical Genetics, Uppsalalaan 8, 3584 CT, Utrecht, Netherlands.
Abstract
Posttranscriptional gene silencing in
Caenorhabditis elegans
results from exposure to double-stranded RNA (dsRNA), a phenomenon designated as RNA interference (RNAi), or from co-suppression, in which transgenic DNA leads to silencing of both the transgene and the endogenous gene. Here we show that single-stranded RNA oligomers of antisense polarity can also be potent inducers of gene silencing. As is the case for co-suppression, antisense RNAs act independently of the RNAi genes
rde-1
and
rde-4
but require the mutator/RNAi gene
mut-7
and a putative DEAD box RNA helicase,
mut-14
. Our data favor the hypothesis that gene silencing is accomplished by RNA primer extension using the mRNA as template, leading to dsRNA that is subsequently degraded.
Publisher
American Association for the Advancement of Science (AAAS)
Cited by
125 articles.
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