Observing the cell in its native state: Imaging subcellular dynamics in multicellular organisms-Reference-Cited by-同舟云学术

Observing the cell in its native state: Imaging subcellular dynamics in multicellular organisms

Published:2018-04-20 Issue:6386 Volume:360 Page:
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Liu Tsung-Li¹^ORCID,Upadhyayula Srigokul¹²³⁴^ORCID,Milkie Daniel E.¹^ORCID,Singh Ved¹,Wang Kai¹,Swinburne Ian A.⁵^ORCID,Mosaliganti Kishore R.⁵,Collins Zach M.⁵^ORCID,Hiscock Tom W.⁵,Shea Jamien¹^ORCID,Kohrman Abraham Q.⁶,Medwig Taylor N.⁶,Dambournet Daphne⁷,Forster Ryan⁷,Cunniff Brian²³^ORCID,Ruan Yuan⁸,Yashiro Hanako⁸^ORCID,Scholpp Steffen⁹¹⁰^ORCID,Meyerowitz Elliot M.⁸^ORCID,Hockemeyer Dirk⁷^ORCID,Drubin David G.⁷^ORCID,Martin Benjamin L.⁶^ORCID,Matus David Q.⁶^ORCID,Koyama Minoru¹^ORCID,Megason Sean G.⁵^ORCID,Kirchhausen Tom¹²³⁴,Betzig Eric¹^ORCID

Affiliation:

1. Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.

2. Department of Cell Biology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA.

3. Program in Cellular and Molecular Medicine, Boston Children’s Hospital, 200 Longwood Avenue, Boston, MA 02115, USA.

4. Department of Pediatrics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA.

5. Department of Systems Biology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA.

6. Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY 11794-5215, USA.

7. Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.

8. Howard Hughes Medical Institute and Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.

9. Living Systems Institute, College of Life and Environmental Sciences, University of Exeter, Exeter EX4 4QD, UK.

10. Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, 76021 Karlsruhe, Germany.

Abstract

Continuing the resolution revolution The living cell contains dynamic, spatially complex subassemblies that are sensitive to external perturbations. To minimize such perturbations, cells should be imaged in their native multicellular environments, under as gentle illumination as possible. However, achieving the spatiotemporal resolution needed to follow three-dimensional subcellular processes in detail under these conditions is challenging: Sample-induced aberrations degrade resolution and sensitivity, and high resolution usually requires intense excitation. Liu et al. combined noninvasive lattice light-sheet microscopy with aberration-correcting adaptive optics to study a variety of delicate subcellular events in vivo, including organelle remodeling during mitosis and growth cone dynamics during spinal cord development. Science , this issue p. eaaq1392

Funder

National Science Foundation

National Institutes of Health

Damon Runyon Cancer Research Foundation

Biogen

Ionis

Howard Hughes Medical Institute

Human Frontier Science Program

Pew Charitable Trusts

Carol M. Baldwin Foundation

Publisher

American Association for the Advancement of Science (AAAS)