Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage

Author:

Jiang Fuguo1,Taylor David W.12,Chen Janice S.1,Kornfeld Jack E.3,Zhou Kaihong3,Thompson Aubri J.4,Nogales Eva1235,Doudna Jennifer A.12345

Affiliation:

1. Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.

2. California Institute for Quantitative Biosciences, University of California, Berkeley, CA 94720, USA.

3. Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA.

4. Department of Chemistry, University of California, Berkeley, CA 94720, USA.

5. Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

Abstract

CRISPR Cas9 molecular scissors The CRISPR-associated (Cas) protein Cas9 is a molecular scissor for cutting DNA. The first step in the cutting reaction is the RNA-guided unwinding of the DNA double helix. Jiang et al. determined the structures of Cas9 bound to DNA unwound by the targeting RNA (see the Perspective by Chen and Bailey). Cas9 bends the DNA to allow guide RNA infiltration into the double helix. The two separated DNA strands, one bound to RNA, are subsequently positioned in the dual active sites of the protein for cutting. Science , this issue p. 867 ; see also p. 811

Funder

Damon Runyon Cancer Research Foundation

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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