Metagenomic evaluation of a Utah tar sand microbiota suggests the predominant hydrocarbonoclastic role of Actinobacteria

Abstract

Background: Occurrences of tar sands have been reported in 22 states in the United States; however, the largest deposit is located in southwestern Utah. It has been suggested that tar sands were created by the microbial degradation of immobile subsurface oil over several million years; however, little is known about the indigenous microbial communities in the bituminous tar sands. Methods: This study identified Utah tar sand microbiota using next-generation sequencing technology and characterized the functional diversity using community-level physiological profile (CLPP). Results: Microbiota identified in these tar sands are mainly affiliated with the Gram-positive Actinobacteria and representatives of genera that have also been previously shown to degrade aromatic hydrocarbons, including Arthrobacter, Dietzia, Janibacter, Nocardioides, Microbacterium, Agrococcus and Salinibacterium, suggesting that these microbes likely play roles in the biodegradation of oil-hydrocarbons. CLPP analysis revealed less than 24 h was needed for the first color development in the microplate wells containing the polymers, whereas the duration of the lag phase of the carboxylic acids was prolonged. Conclusions: The quick utilization of the polymers suggests that the indigenous microbial community, especially the actinomycetes in the tar sand habitat, are poised and primed to degrade these recalcitrant compounds.