Caffeic Acid attenuates acrylamide induced biochemical, hematological and histological alterations in rats

Abstract

Abstract Acrylamide is formed during processing of food at high temperature and is documented as a well-known neurotoxic, carcinogenic and has many adverse effects on human health. Therefore, it is the need of hour to reduce the detrimental effect of acrylamide. In the present sub-chronic study 18 adult Wistar rats were randomly divided into three groups. These groups were normal saline, toxicant, and toxicant (19.13 mg/kg) + therapy (20 mg/kg). Our result showed all the sign and symptoms of acrylamide toxicity which include reduction in the body weight, hind limb splaying, hair loss and skin irritation. A significant alteration in the hematological parameters and sharp enhancement in AST, ALT, ALP, LDH, GGT, urea, uric acid and triglycerides was recorded. Activity of acetylcholinesterase significantly reduced in brain after acrylamide administration. Acrylamide exposure resulted into increased mean DNA damage and tail length in liver, kidney and brain as compared to normal evident by COMET assay. Our biochemical findings were reinforced by histological observation. Treatment with caffeic acid restored tissue and serological indices accompanying towards normal.