Analysis of 26 STRs loci (PowerPlex® Fusion 6C System) in Mestizos from the Mexico City

Abstract

Abstract Background Short tandem repeats (STRs) are the most widely used genetic markers in forensic genetics. Therefore, it is essential to document genetic population data of new kits designed for human identification purposes, in order to enable laboratories to use these genetic systems to interpret and solve forensic caseworks. However, in Mexico, there are not studies with the PowerPlex Fusion 6C System, which included 26 STRs (23 autosomal STRs and 3 Y-STRs). Methods and Results A total of 600 DNA samples from Mexico City were subjected to genotyping using the PowerPlex Fusion 6C System. For autosomal STRs, 321 different alleles were observed, while combined PE and PD were 99.999999809866 and 99.99999999999999999999999818795%, respectively. Genetic distances and AMOVA test showed low but significant differentiation between Mexican populations. Conclusions The results reported in this work demonstrate the efficacy of this system for human identification purposes in the population studied, and justify its possible application in other Mexican Mestizo populations.