Dermatobacter hominis gen. nov., sp. nov., a new member of the family Iamiaceae, isolated from keratin epidermis

Abstract

Abstract A non-motile, novel actinobacterial strain, designated Kera-3T, was isolated on 1/10 diluted R2A agar from human keratinocytes. Strain Kera-3T was Gram-stain-positive, aerobic and rod-shaped. The whole-cell hydrolysis of amino acids contained meso-DAP, alanine, and glutamic acids. The main menaquinone was MK-9 (H8). The major fatty acids were C16:0 and C18:1 ω9c. The major phospholipids were diphosphatidylglycerol and aminophospholipid, along with an unidentified phosphoglycolipid and an unidentified aminophosphoglycolipid. The G + C content of the genomic DNA was 70.5 % based on the complete genome sequence. Phylogenetic analyses of the 16S rRNA gene sequence and core-gene based on amino acid sequence clearly showed that strain Kera-3T formed a new lineage in the family Iamiaceae, being the closest neighbour Rhabdothermincola sediminis SYSU G02662T with a 91.19% 16S rRNA gene sequence identity. Phylogenetic data was supported with a comparative genomic study of the predicted general metabolism and carbohydrate active enzymes. Based on physiological and biochemical characteristics data analysis. Strain Kera-3T can be distinguished from known genera in the family Iamiaceae and represents a novel genus and novel species. Therefore, the name Dermatobacter hominis gen. nov., sp. nov. is proposed with the type strain being Kera-3T (= KACC 22415T = LMG 32493T).