Aptamer-based Colorimetric and Lateral Flow Immunoassay Detection of Aflatoxin B1 in corn Samples.

Abstract

Abstract In this paper, gold nanoparticles were combined with aflatoxin B1-aptamer as a single probe via the specific binding between aflatoxinB1 with aptamer, designing a colorimetric detection method. Meanwhile, an immunochromatographic strip with gold nanoparticles modified by complementary chains of aflatoxin B1 aptamer as the probe was developed for rapid detection of aflatoxin B1. Under the optimal conditions, the linear detection range of the colorimetric method was 0.5–50 ng/mL, the minimum detection limit was 0.5 ng/mL, the detection range of the immunochromatographic strip was 0.5–500 ng/mL, the minimum detection limit was 0.5 ng/ mL, and the semi-quantitative limit of detection (LOD) was 51 ng/mL. Finally, comparing the two methods, it is concluded that immunochromatographic strip detection is more sensitive, accurate and celerity. The recoveries of test samples were from 97.3–120%. All detections could be accomplished within 10 min, indicating that the strip could be a potentially effective tool for rapid on-site detections.