Genome insights from the Amazonian rhizobacterium Bacillus paramycoides RZ3MS14 reveal plant growth-promoting multi-traits and bioprotection against phytopathogens and environmental stresses

Abstract

Abstract Bacillus paramycoides is poorly understood bacterium with potential application as plant growth promoter and agent control bacterium to a more sustainable agriculture. B. paramycoides strain RZ3MS14 was previously isolated from guarana´s rhizosphere on Amazonian rainforest. The obtained RZ3MS14 draft genome comprised 28 scaffolds with a genome size estimated of 5,493,110 bp and a G + C content of 34.9%. We predicted 5,637 coding sequences (CDS). From phylogenomic analysis was observed that RZ2MS14 clustered in a monophyletic group with B. paramycoides strains. The ANI and digital DNA-DNA hybridization comparing RZ3MS14 and the B. paramycoides strain type NH24A2 was 98.5% and 73-80.6% respectively. RZ3MS14 harbors different genes related with plant growth-promoting as instance phosphorus solubilization and mineralization as well as auxin production by IPA pathway and others. Moreover, the RZ3MS14 draft genome presented the putative micrococcin, cerecidin, and paeninodin gene clusters, antibiotics related with pathogen control in addition of genes to VOCs production. Our results contribute to improve the knowledge concerning the molecular basis of B. paramycoides related with its vast application on agriculture.