The need to stabilize the bacterial community present in the cultures of the marine diatom Haslea ostrearia

Author:

Peticca Aurelie1,Fodil Mostefa1,Chenais Benoit1,Sabot Francois2,Casse Nathalie1

Affiliation:

1. University of Le Mans

2. Institut de Recherche pour le Développement

Abstract

Abstract Objectives: Haslea ostrearia is a microalgae associated with the marrenine, a blue pigment known for its economic interest when it attaches to oyster gills. Complex to grow under axenic conditions, H. ostrearia probably needs its own bacterial procession to survive. Consequently, this important bacterial presence greatly complicates any study of its ecological, physiological and genomic content. Results: We therefore tested a first approach of a protocol to control the bacterial concentration without killing the microalgae, by growing four H. ostrearia NCC 532 cultures under two culture conditions: two with and two without antibiotic treatment. We also performed a first bacterial identification method by 16S sequencing adapted to the bacterial community of H. ostrearia. The V3V4 16S region allowed precise identification of bacteria present in H. ostrearia culture. An antibiotic treatment was found as necessary to stabilize the bacterial population of the cultures before the DNA extraction.

Publisher

Research Square Platform LLC

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3