Study on Drip Flow Reaction About Biofilmby Pseudomonas Aeruginosa and Its Application to Evaluate Inhibitory Antibiofilm of Antibiotic

Abstract

Abstract Background: Pseudomonas aeruginosa (Pa) causes nosocomial infections, it has the ability to form biofilms in medical devices leading treatment to complicated. The purpose of this study is to build a biofilm model on Drip Flow Reaction (DFR) system and apply it to investigate the effects of antibiotics on biofilm formation. Materials and method: The ability of Pa ATCC 9027, ATCC 27853 and PA 01 to form biofilms was investigated on DFR. This model is applied to evaluate the biofilm resistance of antibiotics through Flow cytometry (FCM). Results: Successfully built biofilm model of Pa in DFR and obtained images on carrier and results from Flow Cytometry (FCM) machine analysis. The results revealed strains related to biofilm formation, time of antibiotic use is related to biofilm formation, the combination of colistin and rifampicin increased simultaneously both the inhibitory effect on biofilm formation and biofilm destruction (One-Way ANOVA test, p<0.01). Conclusion: The study successfully investigated the biofilm-forming model and applied the Pa biofilm resistance in DRF with colistin and rifampicin.