Response mechanisms of Candida tropicalis incubated with a dilute acid hydrolysate from corn stover

Author:

Zhang zhengyue1ORCID,Wu Lan1,Li Qi1,Shu Baichao1,Li Dang1,Chen Yulei1,Tang Jiaye1,Long Siyi1,Liao Jie1,Zhao Yifan1,Wang Hanyu2,Ma Menggen1

Affiliation:

1. Sichuan Agricultural University - Chengdu Campus

2. Leshan Normal University

Abstract

Abstract Understanding the cellular response mechanisms of Candida tropicalis is crucial to biofuel production from corn stover, and targeted genetic modification of a C. tropicalis fermentation strain can improve the biofuel yield. In this report, metabolomic analysis of a hydrolysate obtained from dilute acid hydrolysis of corn stover identified 1,469 substances, including sugars, aldehydes, acids and phenols. In the presence of the corn stover hydrolysate, cell growth was inhibited. Moreover, subcellular observations revealed that C. tropicalis SHC-03 accumulated reactive oxygen species and maintained endoplasmic reticulum homeostasis when incubated with this hydrolysate. For detoxification of byproducts in the hydrolysate that inhibit cell growth and survival, genes associated with reduced glutathione, ergosterol, and ubiquinone-n biosynthesis and misfolded protein and fatty acid degradation were upregulated upon incubation with the hydrolysate. These results will help guide genetic modifications that increase the intracellular synthesis of NADH/NADPH and acetyl-CoA for ergosterol and fatty acid accumulation to improve tolerance to hydrolytically toxic byproducts and accelerate industrial production of bioethanol and other bioproducts.

Publisher

Research Square Platform LLC

Reference28 articles.

1. Bajpai P (2016) Pretreatment of Lignocellulosic Biomass. In: Pretreatment of lignocellulosic biomass for biofuel production. Springer, Chap. 4, p 17–70

2. Oxidative stress and antioxidant defense;Birben E;World Allergy Organ J,2012

3. Biofuels. Altered sterol composition renders yeast thermotolerant;Caspeta L;Science,2014

4. )Enhanced Production of Xylitol from Poplar Wood Hydrolysates Through a Sustainable Process Using Immobilized New Strain Candida tropicalis UFMG BX 12-a;Dalli SS;Appl Biochem Biotechnol,2017

5. Evaluation of some lignocellulosic byproducts of food industry for microbial xylitol production by Candida tropicalis. 3 Biotech 6;Eryasar K,2016

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3