Abstract
To evaluated the TTD differences of different blood culture bottles (BacT/Alert-PF, BacT/Alert-PF plus, and Bactec-PF) and instruments (BacT/Alert 3D, BacT/Alert VIRTUO, and Bactec FX) through in vitro blood culture by simulating bloodstream infections by different bacterial or fungal species, and we also evaluated the adsorption capacity of three blood culture bottles for various antibiotics through in vitro blood culture collection under simulated peak antibiotic concentrations. Among all tested bacteria, the median TTD of BacT/Alert VIRTUO using BacT/Alert-PF plus and of Bactec FX using Bactec-PF was shortened by 5.63 and 3.77 h, respectively, compared with BacT/Alert 3D using BacT/Alert-PF. Under the same BacT/Alert 3D blood culture instrument, the median TTD of BacT/Alert-PF plus was 3.33 h shorter than that of BacT/Alert-PF. Under the same BacT/Alert-PF plus blood culture bottle conditions, BacT/Alert VIRTUO shortened the median TTD by 2.3 h compared with BacT/Alert 3D. The median TTD of BacT/Alert VIRTUO using BacT/Alert-PF plus was 1.86 h shorter than that of Bactec FX using Bactec-PF. The antibiotic adsorption evaluation test showed that Bactec-PF (51.3%) and BacT/Alert-PF plus (46.1%) had higher detection rates than BacT/Alert-PF (51.3%). The detection rates of Bactec-PF and BacT/Alert-PF plus vary in different combinations of antibiotic strains, and they increase correspondingly with the degree of bacterial resistance. The detection rates of the three blood culture bottles in the amikacin group were all 100%, whereas those in the ceftriaxone and meropenem groups were all 0%. These findings are of great significance to the selection of antibiotics, treatment, and prognosis of bacteremia.