Partial white mold resistance in a Brazilian-adapted common bean panel

Author:

Silva Givanildo Rodrigues1,Gilio Thiago Alexandre Santana1,Gonçalves-Vidigal Maria Celeste2,Araújo Kelly Lana1,Barelli Marco Antonio Aparecido1,Souza Lorenna Lopes2,Neves Leonarda Grillo1,Pastor-Corrales Marcial3

Affiliation:

1. University of Mato Grosso State “Carlos Alberto Ryes Maldonado”

2. State University Maringá

3. Beltsville Agricultural Research Center

Abstract

Abstract

The pathogen Sclerotinia sclerotiorum (Lib.) de Bary is a fungus that causes white mold (WM) in many crops, and it is one of the greatest phytosanitary problems that compromises the productivity and quality of common bean (Phaseolus vulgaris L.). This study aimed to characterize a panel composed of common bean lines (BLs) from Brazilian farmers with WM resistance using two methods/tests under controlled conditions. The “straw test” (ST - Terán et al., 2006) and “seedling straw test” (SST - Arkwazee & Myers, 2017) were used to screen the panel. The disease score (DS) and relative disease progress (RDP) were calculated from consecutive evaluations to obtain the area under the disease progress curve (AUDPC). In addition, the phenotypic means were used to identify genomic regions associated with the WM reaction using the genome-wide association study (GWAS) approach. In total, fifteen accessions (eleven Mesoamerican and four Andean) were selected showing high to moderate resistance, and three regions were identified on chromosomes Pv01, Pv02 and Pv03, coinciding with previously reported quantitative trait loci (QTLs), additionally, twelve genes were indicated for validation. We identified putative regions and genes contributing to physiological resistance to WM in a well-adapted common bean panel. The regions indicated in this panel that are adapted to the Brazilian climate may be important in common bean breeding programs.

Publisher

Springer Science and Business Media LLC

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