Growth, intestinal health, nutritional profile and molecular mechanism of the hydrid grouper, E. lanceolatus♂× E. fuscoguttatus♀juveniles reared with microalgae Chlorella pyrenoidosa

Abstract

Abstract The effects of microalgae Chlorella pyrenoidosaon the hybrid groupers’ (Epinephelus lanceolatus♂×E. fuscoguttatus♀) growth, gut microbiome and transcriptome were examined in this study. Feeding trials with duration of 15-days (15d) and 60-days (60d) were conducted on three experimental groups (n=3) as follow; (T1) grouper fed with basal diet and reared in filtered seawater (control treatment), (T2) grouper fed with basal diet and reared in C. pyrenoidosa monoculture water and (T3) grouper fed with basal diet partially replaced with 15% of dried C. pyrenoidosa (CRM) and reared in filtered seawater. The findings revealed that groupers reared 15d in T2 treatment (T2-15d) had a higher fat content, with apparent shift of microbial composition and functional pathways in the gut. Groupers reared 60d in T2 treatment (T2-60d) displayed an increased NADH dehydrogenases and cytochrome c oxidases gene expression, indicating more robust oxidative phosphorylation activity and ATP production crucial for the metabolic homeostasis. The reduction in the total amino acid content was also detected in groupers raised in T2-60d. Gastritis, enteritis and lipid malabsorption syndrome were observed in groupers raised 60d in T3 treatment (T3-60d), with the condition likely due to a higher intake of omega-6 to omega-3 fatty acids ratio in grouper raised 15 days in T3 (T3-15d) and T3-60d. This result indicated that grouper intestinal inflammation could arise from the inclusion of dried C. pyrenoidosa. Overall, the study outcomes demonstrated that introducing live C. pyrenoidosa to the culture water is advantageous in the digestion and enhance the energy metabolism of juvenile groupers.